《植物生理学报》 2015, 51(3): 323-336

天冬氨酸蛋白酶在拟南芥和水稻中的分子进化、表达模式以及在花药发育中的功能分析

汤龙军^1,2, 李鹏程², 朱璐², 王瑞丰², 陈旭², 李宗芸¹, 许杰^2,*

¹江苏师范大学生命科学学院, 江苏徐州221000; ²上海交通大学生命科学技术学院, 上海200240

通信作者：许杰；E-mail: xujie3000@sjtu.edu.cn；Tel: 021-34204869

摘　要：

天冬氨酸蛋白酶(aspartic proteases, AP_s)是一类功能多样化的酶, 参与了植物大部分生理和发育过程, 但其基因家族在植物中的系统分类、分子进化以及功能方面研究仍很欠缺。本研究收集整理了拟南芥和水稻中AP_s基因序列、分类、基因结构和染色体定位等信息, 对比分析该基因家族在拟南芥和水稻中分子进化方面的差异; 利用转录组数据阐明该家族基因在不同组织部位和不同发育时期的表达差异和功能分化。此外, 本文还围绕花药发育的过程, 细致分析了各亚家族AP_s基因的表达变化, 推测AP_s在花药发育过程中的可能功能。

关键词：天冬氨酸蛋白酶; 分子进化; 功能分化; 花药

收稿：2015-01-04   修定：2015-01-30

资助：国家自然科学基金面上项目(31000593、31370026和31271698)。

Identification, Evolutionary and Expression Profile Analysis of the Aspartic Protease Gene Superfamily in Arabidopsis thaliana and Rice

TANG Long-Jun^1,2, LI Peng-Cheng², ZHU Lu², WANG Rui-Feng², CHEN Xu², LI Zong-Yun¹, XU Jie^2,*

¹School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu 221000, China; ²School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: XU Jie; E-mail: xujie3000@sjtu.edu.cn; Tel: 021-34204869

Abstract:

Aspartic proteases (AP_s), a large family of proteolytic enzymes with diverse functions, play important roles in many aspects of physiological and developmental processes in plants. However, systematic studies on their classification, molecular evolution and function are still lacking. In this study, information about Aps including gene sequence, subfamily classification, gene structure, sub-cellular localization and chromosomal location of all known APs in Arabidopsis and rice were collected, and analyzed by bioinformatics tools. In addition, the difference in spatio-temporal expression and function of these APs between Arabidopsis and rice was investigated using available trasnscriptomic data. Furthermore, the expression pattern of each of the AP_s genes along anther development was highlighted, which provides a framework for future studies defining the roles of AP_s in anther development.

Key words: aspartic protease; molecular evolution; functional differentiation; anther